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So far you have tested the effect of substrate (H₂O₂) concentration on the catalase reaction. Plastic Cups, 16 oz (5). To show the similarity between enzymes and chemical catalysts, the teacher may wish to demonstrate (or ask the class to perform as part of the class experiment) the catalytic decomposition of hydrogen peroxide solution by manganese(IV) oxide (HARMFUL – see CLEAPSS Hazcard HC060). You should cite outside resources. This experiment combines heat, jello, and pineapple to observe how temperature affects reactions. For example, proteases (enzymes that break peptide bonds in proteins) will not work on starch (which is broken down by the enzyme amylase). Describe the specific action of the enzyme catalase, include the substrate and products of the reaction. Temperature hypothesis. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. Enzyme catalase lab report. There were four more test tubes that were included in the procedure.
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Enzyme Catalase Lab Report

Decreases enzyme activity. Identify substrate, enzyme and products. Optional: Food coloring. To cup 4, add 2 tablespoons of 3% hydrogen peroxide. In this science activity you will investigate one of these enzymes, called catalase, to find out how it helps to protect your body from cell damage. The enzyme catalase, found in potato juice, was used for the catalyst along with a substrate known as hydrogen peroxide (H2O2). Health, safety and technical notes. Enzymes speed the rate of chemical reactions. This is a resource from the Practical Chemistry project, developed by the Nuffield Foundation and the Royal Society of Chemistry. Catalase enzyme lab answer key west. In case you spill hydrogen peroxide, clean it up with a wet paper towel.

Catalase Enzyme Lab Answer Key For Teachers

To prevent such damage, the catalase enzyme helps getting rid of these compounds by breaking up hydrogen peroxide into harmless water and oxygen (Figure 2). • To test the effect of heat, put one teaspoon of the blended liver into a microwave-safe bowl. Add 10 drops of distilled water to the tube marked B. How do the results in each cup look different? 13 Enzymes Lab Report Activities. Do they work as well as yeast? Do not discuss why something occurred, just state what occurred. As pH comes closer to a neutral pH the enzyme is at its greatest effectiveness. Investigation of catalase activity in plant and animal tissues. • Raw liver (fresh or frozen, thawed; one quarter pound). Enzymes are biological catalysts which increase the speed of a chemical reaction.

Catalase Enzyme Lab Answer Key West

One test tube was put in ice and the other in warm water. You should have three procedures, one for each hypothesis. The height of the foam is an indication of the amount of catalase activity present. When I first started doing this lab I used petri dishes for all the potato purees and it was a lot of clean up. Repeat this experiment, but this time do not add dish soap to all of the reactions.

However, at some point you will reach a substrate concentration at which the enzyme gets saturated and becomes the limiting factor. How Heat Affects Enzymes. Hydrogen peroxide decomposes slowly in light to produce oxygen and water. It was hypothesized that as the temperature increased, the reaction rate would increase.

Also at the absorbance of a slope of 0. Conical flasks, 100 cm3, x3. Obtain two test tubes and label one as A and one as B. Is the process faster or slower when compared to the control experiment conducted at room temperature? The compounds or molecules the enzyme reacts with are called their substrates.

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