The water should not be too hot but close to body temperature (37°C). The enzyme catalase, found in potato juice, was used for the catalyst along with a substrate known as hydrogen peroxide (H2O2). In this case you have to add more enzyme to speed up the reaction again. Catalase enzyme lab answer key.com. Under which conditions does the enzyme work well, and under which ones does it work poorly? Tube 2 at room temperature (record the temperature). Record your results—you may want to draw tables.
Students will look at enzyme reaction rates, how enzymes react with substrate concentration and the effect of temperature on enzyme reactions. Typically this section is written in first person past tense in paragraph form since you conducted the experiment. In fact, there are thousands of different enzymes in your body that work around-the-clock to keep you healthy and active. The pH effect on enzymes can be tested by trying each pH level with a pH buffer of the same pH as labeled as the test tube and 1mL of potato juice, water, and catechol. Firstly, students will explore important concepts about enzymes; including what enzymes are, how they help cells, and how they create reactions. The dependent variable changes in response to the experimental variable. Safely dispose of any raw liver meat used in this activity by putting it in the trash when you are done. Place a beaker of peroxide in an ice bath, and another in a warm water bath. Introduction: Starting out with some background information, I know that enzymes are biological catalysts. Catalase enzyme lab answer key images. This energy is called the activation energy. If you have an excess of enzyme but not enough substrate, the reaction will be limited by the substrate availability. This experiment combines heat, jello, and pineapple to observe how temperature affects reactions. Add the appropriate extract to each tube to the level of the 1 cm line. Sometimes they get weird data, so this helps with accuracy.
Add extract to the 1 cm line. Many other factors affect the activity of enzymes as well. 494) affects the enzyme more than a pH of 4 (0. Place the paper in the bottom of the beaker of peroxide and start the stopwatch. Catalase is a very common enzyme that is present in almost all organisms that are exposed to oxygen. Schematic drawing of an enzyme reacting with its substrate. • Put one drop of the blended liver on the large plate. Recent flashcard sets. Exploring Enzymes | STEM Activity. Critical Thinking With Enzymes. If we had continued to test out different concentrations of our substrate, the reaction time would have continued to decrease until the enzyme hit what is known as the saturation level, which means all the enzymes are working and the reaction cannot continue to increase because there is no location at that time for the substrates to bind to. Fill each of two test tubes with catalase (from the potato) to the 1 cm mark. Optional: Food coloring.
If the results matched your predictions, hypothesize some mechanisms behind what you have observed. Is the result different or the same compared to cup number 2? Once students complete the lab, there are also several activities for extension learning. Hold a glowing splint in the neck of each flask. The motive of this lab is to attain a better understanding of enzyme activity by timing chemical reactions in certain temperatures and pH levels. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. There were four more test tubes that were included in the procedure. Warning is hereby given that not all Project Ideas are appropriate for all individuals or in all circumstances. Catalase enzyme lab answer key hgb 3 27 2000. Learning about enzymes is important to build basic skills and an understanding of biological processes. Students will read A Journey Through the Digestive System and Amazing Body Systems: Digestive System, along with watching several videos in order to prepare to discuss how enzymes aid in digestion and the cleaning of clothes.
Before using the hydrogen peroxide, put on your safety goggles to protect your eyes. Or you could try adding more than one teaspoon of vinegar or baking soda and then test the enzyme. Learn more: The Homeschool Scientist. What is the color and consistency of this mixture? Catalase Enzyme Lab Flashcards. Kids will use food coloring, yeast, dish soap, and hydrogen peroxide to simulate how enzymes react in the body. As pH comes closer to a neutral pH the enzyme is at its greatest effectiveness.
This is another online activity that challenges students to see the similarities between using a penny machine and the enzymatic process. Prepare a bar graph of catalase activity. Then, students repeat the experiment by changing the temperature of either the potato puree or the hydrogen peroxide to measure the effect of temperature on catalase. In this label we will be testing different pH levels and temperatures to see which ones cause the most reactions. What Effects of Concentrations of a Substrate on Enzyme Lab Answers. Temperature hypothesis. Different tissues in the body have different pHs (pH is a measure of how basic or acidic a solution is). We will also test how this process is affected by changes in the temperature of the potato. Using a potato and hydrogen peroxide, we can observe how enzymes like catalase work to perform decomposition, or the breaking down, of other substances. Write your procedure to test each hypothesis. Plant and Animal Enzyme Lab. During the lab, students will be able to identify the sugars in each type of milk.
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