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Virtually all liquid gases present the threat of poisoning, explosion, or, at a minimum, asphyxiation in a confined space. Water will not readily extinguish such fires; instead, it can cause the fire to spread or float to adjacent areas. Lab scenes in movies. Never use a screwdriver to pry off a stuck cap or pliers to open a cylinder valve. However, never use oil or lubricant on any equipment that will be used with oxygen. Wear appropriately rated safety glasses, chemical splash goggles, and face shields for eye protection.
The speed of an induction motor is determined by the AC frequency rather than the voltage. They are normally fitted with a male plug that fits into a female receptacle on an output line from a variable autotransformer. • Replace gloves immediately if they are contaminated or torn. Model 2 scenes in the lab answers. Before beginning any work, all personnel should be shown and trained in the use of all electrical power sources and the location of emergency shutoff switches. There was a warning decal on the unit explaining which model rotors were acceptable.
The sealed glass tubes can be placed either inside pieces of brass or iron pipe capped at one end with a pipe cap or in an autoclave containing some of the reaction solvent (to equalize the pressure inside and outside the glass tube). Do not transfer liquefied gases from one container to another for the first time without the direct supervision and instruction of someone who is experienced in this operation. Place cylinders so that the rotary cylinder valve handle at the top is accessible at all times. Machine warning lights indicate when the beam shutter is open. Safety first model 2 scenes in the lab answers. Do not use ovens to dry any chemical sample that has even moderate volatility and might pose a hazard. Valves should be either completely open or completely closed. The laboratory supervisor is responsible for ensuring that all personnel are aware of the locations of fire extinguishers and are trained in their use. These mantles enclose a heating element in layers of fiberglass cloth. Check regulators before use to verify they are free of foreign objects and to correct for the particular gas.
Molten salt baths, like hot oil baths, offer the advantages of good heat transfer, commonly have a higher operating range (e. g., 200 to 425 °C), and may have a high thermal stability (e. g., 540 °C). Use with the specific cylinder, and use adapters only on the downstream side of the regulator. Other physical hazards include electromagnetic radiation from lasers and radio-frequency generating devices. If a spill exceeds the on-scene personnel's ability or challenges their safety, they should leave the spill site and call the emergency telephone number for help. Use only round-bottom or thick-walled (e. g., Pyrex) evacuated reaction vessels specifically designed for operations at reduced pressure. The most commonly used drying agents involve potentially pyrophoric metals: sodium metal/benzophenone and magnesium metal/iodine. Trained laboratory personnel should be familiar with, and have in place, alternative power shutoffs (i. e., properly installed crash buttons, ready access to equipment power cord plugs). A brush and dustpanHow should you dispose broken glass? The potential hazards posed by laboratory refrigerators include release of vapors from the contents, the possible presence of incompatible chemicals, and spillage. • Minimize condensation that may enter electrical equipment if it is placed in a cold room or a large refrigerator. Microwave ovens can heat material (e. g., solidified agar) so quickly that, even though the container lid is loosened to accommodate expansion, the lid can seat upward against the threads and the container can explode. If there is any risk of exposure, call the environmental health and safety office and evacuate the area before the tank is moved. Each situation needs to be evaluated individually.
To ensure that the equipment has been properly designed, each pressure vessel should have stamped on it, or on an attached plate, its maximum allowable working pressure, the allowable temperature at this pressure, and the material of construction. Wear proper protective gloves when handling hazardous chemicals, toxic materials, materials of unknown toxicity, corrosive materials, rough or sharp-edged objects, and very hot or very cold objects. 3. turn off fume hoods. Their use in filtration or solvent-removal operations involving volatile organic solvents presents a hazard that volatile chemicals will contaminate the wastewater and the sewer, even if traps are in place.
2 Liquefied Gases and Cryogenic Liquids. This risk can be avoided by wiring a fusible coupling into the line inside the hot plate. High-voltage and high-power requirements are increasingly prevalent; therefore prudent practices for handling these devices are increasingly necessary. Take every precaution to prevent injury from flying glass or from corrosive. • Summon medical help immediately.
Computer equipment may need to be replaced if exposed to sufficient amounts of the dry powders. In a system protected by a rupture-disk device, the maximum operating pressure should be approximately two-thirds of the rated MAWP; the exact figure is governed by the fatigue life of the disk used, the temperature, and load pulsations. Direct sunlight can damage eyes, use a desk lamp instead. This means running some 20 loads through industrial dishwashers every day, oven-baking or autoclaving the items to sterilise them – and clocking up a lot of steps to pick them up and return them. Transfer very slowly to minimize boiling and splashing. Remove regulators from corrosive gases immediately after use and flush with dry air or nitrogen. Stuffing boxes and gland joints are a likely source of trouble in pressure installations. NFL NBA Megan Anderson Atlanta Hawks Los Angeles Lakers Boston Celtics Arsenal F. C. Philadelphia 76ers Premier League UFC. This type of construction minimizes the risk of electric shock and of accidentally producing an electrical spark near a flammable liquid or vapor (see Chapter 6, section 6. With the use of liquid hydrogen particularly, explosive conditions may develop. Only trained laboratory personnel should use and service this equipment. It should have a quick-opening valve requiring manual closing; a downward-pull delta bar is satisfactory if long enough. In many localities conserving water is essential and makes tap water inappropriate.
Perform inspections of emergency equipment as follows: • Inspect fire extinguishers for broken seals, damage, and low gauge pressure (depending on type of extinguisher). Use safety shields for protection against possible explosions or splash hazards. Lab workers had left samples running unattended in an ultracentrifuge using a large aluminum rotor that previously had been used multiple times without incident. Glass components of the rotary evaporator should be made of Pyrex or similar glass. Heating baths associated with these devices (e. g., baths for rotary evaporators) should also be spark-free and controllable from outside the hood. For the same reason, fit oil baths left unattended with thermal-sensing devices that turn off the electric power if the bath overheats. In addition, oxygen, hydrogen, and helium are often used in the liquid state. Perform leak checks periodically. Only leak-test solutions Specifically designed for oxygen compatibility may be used to test for oxygen leaks; do not use soap solutions because they may contain oils that can react violently with the oxygen. General-purpose laboratory vacuum pumps should have a record of use to prevent cross-contamination or reactive chemical incompatibility problems. When effective engineering controls are not possible, use suitable respiratory protection after proper training. Because this practice does not always prevent binding of the impeller, it is also desirable to fit unattended stirring motors with a suitable fuse or thermal protection device.
For any reaction run on a large scale (>10 g total weight of reactants) or at a maximum pressure in excess of 690 kPa (100 psi), use only procedures involving a suitable high-pressure autoclave or shaker vessel. Do not use glass vessels with angled or squared edges in vacuum applications unless specifically designed for the purpose (e. g., extra thick glass). The safety shielding within the unit did not contain all of the metal fragments. Also see section 7. ) • Do not overfill the centrifuge tubes. Locate receptacles that provide electric power for operations in laboratory chemical hoods outside the hood. Fail-safe devices, which can be either purchased or fabricated, can prevent the more serious problems of fires or explosions that may arise if the temperature of a reaction increases significantly because of a change in line voltage, the accidental loss of reaction solvent, or loss of cooling.
Glassware under vacuum is at risk for implosion, which could result in flying glass. "The most satisfying aspect of working at the Crick is knowing what I do could help researchers now, or in the future, discover cures for disease", she says. Prudent practices require posting warnings, cordoning off the area at the 5-G line, and limiting access to areas with more than 10 to 20 G to knowledgeable staff. Stills in use should be attended at all times and should have an automatic high-temperature shutoff. Check and clean chemical cartridge respirators on a regular basis. Copper tubing hardens and cracks on repeated bending. Care must be taken to ensure that, in the event of failure, this relief device is oriented away from personnel. If a slight positive pressure of gas on these lines is desired, the recommended pressure range is not in excess of 1 to 2 psi. The impact of exposure to toxic substances can be aggravated when a person inserts his or her head inside a refrigerator to search for a particular sample. Protective equipment is not fail-safe and should not be relied on to prevent cutting injuries. Vacuum work can be carried out on a Schlenk line, an apparatus used for work with air-sensitive compounds, as long as proper technique is used. Personnel who are at risk for lifting injuries should receive periodic training. Remove all mercury from the cold oven with the use of appropriate cleaning equipment and procedures (see Chapter 6, section 6. A flame or any heat sourceWhy shouldn't you remove chemicals or other materials from the laboratory area?
Generally, select a gauge with a range that is double the working pressure of the system. Although a vacuum distillation apparatus may appear to provide some of its own protection in the form of heating mantles and column insulation, this is not sufficient because an implosion could scatter hot flammable liquid. All pressure regulators should be equipped with spring-loaded pressure-relief valves (see section 7.